Research Articles
Validating AFM Stiffness Measurements: A Multimodal Framework for Robust Biomaterial and Cell Mechanobiology (2025)
Atomic Force Microscopy (AFM) is the dominant technique for nanomechanical characterization in biomedicine, yet validating its measurements is paramount for reliability in research and drug development.
Probing the Nanoscale: Single-Molecule Force Spectroscopy with AFM to Decipher Biofilm Adhesins
This article explores the transformative role of Atomic Force Microscopy (AFM)-based single-molecule force spectroscopy (SMFS) in quantifying the nanoscale forces governing biofilm adhesion.
Nanomechanical Mapping of Biofilms: A Comprehensive Guide to AFM Force Volume Technique for Elasticity Analysis
This article provides a comprehensive overview of the Atomic Force Microscopy (AFM) Force Volume technique for mapping the nanomechanical properties of bacterial biofilms.
Chemical Force Microscopy: Probing Microbial Surface Properties for Drug Development and Biomedical Research
Chemical Force Microscopy (CFM) has emerged as a powerful technique in microbiological research, enabling the nanoscale mapping of chemical and physical properties of microbial surfaces under physiological conditions.
Visualizing Dynamic Biofilm Processes: How High-Speed AFM is Revolutionizing Microbiology and Drug Development
This article explores the transformative role of High-Speed Atomic Force Microscopy (HS-AFM) in visualizing the dynamic processes of biofilm formation and behavior in real-time.
Single-Cell Force Spectroscopy in Bacterial Adhesion: Protocols, Applications, and Future Directions for Antimicrobial Research
This comprehensive review explores single-cell force spectroscopy (SCFS) as a transformative biophysical technique for quantifying bacterial adhesion forces at the single-cell and single-molecule level.
Targeting Metabolic Heterogeneity in Persister Cells: From Mechanisms to Therapeutic Breakthroughs
Persister cells, a dormant subpopulation in both bacterial and cancer contexts, exhibit profound metabolic heterogeneity, which is a key driver of antibiotic and chemotherapy treatment failure.
Nutrient Gradients and Bacterial Persistence: Mechanisms, Models, and Therapeutic Targeting in Biofilms
This article synthesizes current research on how nutrient gradients within biofilms drive the formation of antibiotic-tolerant persister cells, a major cause of chronic and recurrent infections.
Stochastic vs. Triggered: Decoding the Dual Mechanisms of Bacterial Persister Cell Formation
This article provides a comprehensive analysis of the two primary paradigms in bacterial persister cell formation: stochastic, internal fluctuations and triggered, external stress-response pathways.
SOS Response and Antibiotic Tolerance: Mechanisms, Therapeutic Targeting, and Future Directions
This article provides a comprehensive analysis of the complex relationship between the bacterial SOS response and antibiotic tolerance for researchers and drug development professionals.