Research Articles
qPCR vs. ddPCR for Absolute Bacterial Quantification: A Strategic Guide for Biomedical Researchers
This article provides a comprehensive comparison of quantitative PCR (qPCR) and droplet digital PCR (ddPCR) for the absolute quantification of bacteria in complex samples.
Viability Assessment in Biomedicine: A Critical Comparison of Plate Culture and Modern Molecular Techniques
This article provides a comprehensive analysis of cell viability assessment methods, contrasting traditional plate culture with advanced molecular techniques.
Comparative Analysis of Bacterial Viability Methods: From Classic Culturability to Advanced Molecular Assays
This article provides a comprehensive comparative analysis of bacterial viability assessment methods, tailored for researchers, scientists, and drug development professionals.
Shotgun Metagenomics vs. 16S rRNA Sequencing: A Strategic Guide for Microbial Community Profiling in Drug Discovery
This article provides a comprehensive comparison of 16S rRNA gene sequencing and shotgun metagenomics for microbial community profiling, tailored for researchers and drug development professionals.
Traditional vs. Molecular AST Methods: A Comprehensive Guide for Research and Diagnostic Applications
This article provides a comparative analysis of traditional phenotypic and modern molecular Antimicrobial Susceptibility Testing (AST) methods for researchers, scientists, and drug development professionals.
Benchmarking Host Gene Expression Signatures: Performance, Applications, and Optimization in Biomedicine
Host gene expression signatures (GES) are powerful tools for discriminating infection types, predicting disease severity, and driving drug repurposing.
Bruker vs. Zybio MALDI-TOF MS: A 2025 Comparative Analysis for Clinical and Research Microbiology
This article provides a comprehensive comparison of Bruker and Zybio MALDI-TOF MS systems, leveraging the latest 2025 research and FDA clearances.
Advanced Strategies for Culturing Stressed Bacteria: From VBNC States to Clinical and Biotechnological Applications
This article provides a comprehensive guide for researchers and drug development professionals on overcoming the critical challenge of bacterial unculturability under stress.
Optimizing Spike-in Internal Reference Quantification: A Complete Guide from Foundations to Validation
Spike-in normalization represents a powerful approach for accurate quantification in genomic assays, particularly when global changes in DNA-associated proteins or transcript abundance occur between samples.
Absolute Quantification of Low-Biomass Microbiomes: A Complete Guide for Robust Research and Clinical Application
This article provides a comprehensive guide for researchers and drug development professionals on achieving absolute quantification in low-biomass microbiome studies.