Research Articles
Mastering pH Control in Fluorescein Diacetate Assays: A Complete Guide for Reliable Viability and Activity Measurement
This comprehensive guide addresses the critical challenge of pH sensitivity in fluorescein diacetate (FDA) assays, which are widely used for assessing cell viability and enzymatic activity in biomedical research and...
Persister Cell Resuscitation: Mechanisms, Methods, and Therapeutic Implications
This article provides a comprehensive analysis of bacterial persister cell resuscitation, a critical process underlying chronic and relapsing infections.
The Biofilm Matrix: Decoding the Diffusion Barrier and Its Role in Antibiotic Treatment Failure
Biofilms represent a fundamental mode of bacterial life that confers a remarkable level of protection against antimicrobial agents.
Beyond the Static State: Advanced Strategies for Assessing Viability in Metabolically Dormant Cells
Metabolically dormant cells—found in contexts from cancer recurrence to bacterial persistence—represent a significant challenge across biomedical research and therapeutic development.
Targeting Persistent Bacterial Subpopulations: Innovative Strategies for Antibiotic Dose Reduction
This article comprehensively reviews the challenge of bacterial persisters—dormant, non-growing cells that survive antibiotic treatment and cause chronic, relapsing infections.
Overcoming the VBNC Challenge: Advanced Detection Strategies for Resistant Pathogens
The viable but non-culturable (VBNC) state is a critical survival strategy adopted by numerous bacterial pathogens in response to stress, rendering them undetectable by conventional culture methods while maintaining metabolic...
Optimizing Periodic Antibiotic Dosing to Eradicate Biofilms: A Research and Development Guide
Biofilms are a primary cause of chronic, recalcitrant infections, exhibiting extreme tolerance to conventional antibiotic regimens.
Metabolic Activity Dyes for Bacterial Viability Assessment: A Comprehensive Guide for Researchers
This article provides a comprehensive overview of metabolic activity dyes for assessing bacterial viability, a critical technique for researchers, scientists, and drug development professionals.
Droplet Digital PCR for Low Biomass Bacterial Quantification: A Comprehensive Guide for Researchers and Drug Developers
This article provides a comprehensive overview of droplet digital PCR (ddPCR) for the precise and absolute quantification of bacterial species in low-biomass samples.
Flow Cytometry for Bacterial Viability Assessment: A Comprehensive Guide for Modern Microbiology
This article provides a comprehensive overview of flow cytometry (FCM) for bacterial viability assessment, a powerful tool offering rapid, single-cell analysis that surpasses traditional culture-based methods.